| Specifications | 57-12-19 | 54-10-12 | 54-12-8 | Comments |
| Numerical Aperture | 0.30 @ RI 1.53 | 0.40 @ RI 1.45 | 0.70 @ RI 1.45 | NA proportional to RI |
| Immersion Media RI | 1.33 – 1.56 | 1.33 – 1.56 | 1.33- 1.56 | Includes all major clearing solutions |
| Effective Focal Length | 19.6 mm @ RI 1.53 | 12.0 mm @ RI 1.45 | 8.4 mm @ RI 1.45 | Magnification proportional to RI |
| Working Distance | 18.1 mm (for all RI) | 12 mm (for all RI) | 10.0 mm (for all RI) | |
| Flat Sample Depth | 8.2 mm | 5.1 mm | 2.0 mm | Imaging depth into flat sample at 45 degree tilt |
| Field of View | 3.0 mm Ø | 1.2 mm Ø | 1.0 mm Ø | |
| Spherical Correction | 480 – 900 nm | 480 – 1000 nm | 480 – 1300 nm | Diffraction-limited for most media and λ |
| Chromatic Correction | 480 – 720 nm | 480 – 720 nm | 480 – 720 nm | Performance varies by media, optimized for CLARITY and TDE |
| Correction Collar | None | None | None | Designed for immersion w/o coverslip |
| Parfocal Length | 75.0 mm | 61.6 mm | 83 mm | Both have M25 threads |
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